Developmentally transient CB1Rs on cerebellar afferents suppress afferent input, downstream synaptic excitation, and signaling to migrating neurons.

J Neurosci. 2020 Jul 06;:

Authors: Barnes JL, Mohr C, Ritchey CR, Erikson CM, Shiina H, Rossi DJ

Abstract
The endocannabinoid system plays important roles in brain development, but mechanistic studies have focused on neuronal differentiation, migration, and synaptogenesis, with less attention to trans-cellular interactions that coordinate neurodevelopmental processes across developing neural networks. We determined that in the developing rodent cerebellar cortex (of both sexes), there is a transient window when the dominant brain cannabinoid receptor, CB1R, is expressed on afferent terminals instead of output neuron Purkinje cell synapses that dominate the adult cerebellum. Activation of these afferent CB1Rs suppresses synaptic transmission onto developing granule cells, and consequently also suppresses excitation of downstream neurons in the developing cortical network, including non-synaptic, migrating neurons. Application of a CB1R antagonist during afferent stimulation trains and depolarizing voltage steps caused a significant, sustained potentiation of synaptic amplitude. Our data demonstrate that transiently expressed afferent CB1Rs regulate afferent synaptic strength during synaptogenesis, which enables coordinated dampening of transcortical developmental signals.Significance StatementThe endogenous cannabinoid system plays diverse roles in brain development, which combined with the rapidly changing legal and medical status of cannabis related compounds, makes understanding how exogenous cannabinoids affect brain development an important biomedical objective. The cerebellum is a key brain region in a variety of neurodevelopmental disorders, and the adult cerebellum has one of the highest expression levels of CB1R, but little is known about CB1R in the developing cerebellum. Here we report a developmentally distinct expression and function of CB1R in the cerebellum, in which endogenous or exogenous activation of CB1Rs modifies afferent synaptic strength and coordinated downstream network signaling. These findings have implications for recreational and medical use of exogenous cannabinoids by pregnant and breastfeeding women.

PMID: 32631938 [PubMed – as supplied by publisher]


Source: ncbi 2

Partage le savoir
Categories: Medical

error: Content is protected !!