Related Articles

A new Cannabis genome assembly associates elevated cannabidiol (CBD) with hemp introgressed into marijuana.

New Phytol. 2021 Feb 01;:

Authors: Grassa CJ, Weiblen GD, Wenger JP, Dabney C, Poplawski SG, Motley ST, Michael TP, Schwartz CJ

●Demand for cannabidiol (CBD), the predominant cannabinoid in hemp (Cannabis sativa), has favored cultivars producing unprecedented quantities of CBD. We investigated the ancestry of a new cultivar and cannabinoid synthase genes in relation to cannabinoid inheritance. ●A nanopore-based assembly anchored to a high-resolution linkage map provided a chromosome-resolved genome for CBDRx, a potent CBD-type cultivar. We measured cannabinoid synthase expression by cDNA sequencing and conducted a population genetic analysis of diverse Cannabis accessions. Quantitative trait locus mapping of cannabinoids in a hemp x marijuana segregating population was also performed. ●Cannabinoid synthase paralogs are arranged in tandem arrays embedded in long terminal repeat retrotransposons (LTR-RT) on chromosome 7. Although CBDRx is predominantly of marijuana ancestry, the genome has cannabidiolic acid synthase (CBDAS) introgressed from hemp and lacks a complete sequence for tetrahydrocannabinolic acid synthase (THCAS). Three additional genomes, including one with complete THCAS confirmed this genomic structure. Only cannabidiolic acid synthase (CBDAS) was expressed in CBD-type Cannabis, while both CBDAS and THCAS were expressed in a cultivar with an intermediate THC:CBD ratio. ●Although variation among cannabinoid synthase loci might affect the THC:CBD ratio, variability among cultivars in overall cannabinoid content (potency) was also associated with other chromosomes.

PMID: 33521943 [PubMed – as supplied by publisher]

Source: ncbi

Partage le savoir
Categories: Medical

error: Content is protected !!